Volcano plot comparing the number of significantly hyper- and hypomethylated probes in patients with FLT3-ITD. Mean methylation differences (x-axis) and negative log10-transformed statistical P values (y-axis) between AML with and without FLT3-ITD are shown in the volcano plot. Methylation levels of the 2501 CpG sites were significantly different between the groups at false discovery rate–adjusted P values <.05, which are represented in blue. Dots in other colors correspond to various genes as follows: brown, NKX2-3; white, HOXB2; aquamarine, HOXB4; green, HOXB5; cyan, HOXB6; black, FLT3; red, HIST1H genes (including HIST1H2AE, HIST1H2AJ, HIST1H2BG, HIST1H2BI, HIST1H3E, and HIST1H3J); orange, PRDM16; purple, HOXB3; and pink, HOXA4. Among the 12 dots of PRDM16, 1 CpG site appeared on the hypomethylation side located near the TSS of PRDM16. In contrast, the remaining 11 CpG sites appeared on the hypermethylation side located in the PRDM16 gene body.