Strong associations of DNA methylation profiles with PRDM16 expression. (A) Heatmap of the DNA methylation profiles based on the methylation values of 244 CpG sites associated with PRDM16 expression. Arrangement of the 64 patients according to a series of PRDM16 expression values (more rightward column location indicates stronger PRDM16 expression) yielded 2 groups with distinct methylation patterns. This classification substantially distinguished patients with high and low PRDM16 expression. (B) Heatmap of 30 CpG sites associated with particularly large differences in methylation values between patients with high and low PRDM16 expressions. (C) Chromatin state annotations for the PRDM16 gene across 127 reference epigenomes based on the National Institutes of Health Roadmap project and chromatin accessibility of the PRDM16 gene in 5 patients with pediatric AML. Chromatin status is indicated by color information, such as purple for bivalent chromatin and gray for repressed polycomb. Supplemental Figure 9 provides details. Only 10 CpG sites among the 649 array probes corresponding to PRDM16 exhibited significant differences in methylation according to PRDM16 expression levels. (D) TSSs of SCHIP1 in which 4 CpG sites were included in the 244 CpG sites opened specifically in the 2 AMLs with high PRDM16 expression.