VHL deletion induces γ-globin through the activation of HIF1α. (A-B) Deletion of VHL in HuDEP2 cells using sgRNA-1 (g1) or sgRNA-4 (g4) induces HbF, a finding that is abolished by concomitant deletion of HIF1α. (C) γ-globin, VHL, and HIF1α mRNA levels were analyzed by quantitative reverse transcriptase-polymerase chain reaction. Data are shown as means ± standard deviation from 3 experiments (**P < .01; ***P < .001; n.s., not significant; unpaired Student t test).