Figure 3.
ICH first occurs in the ganglionic eminence and progresses to ventricle and cortical involvement. (A-D) Representative confocal images from thick sections of Nfe2+/+ (n = 3 per stage) and Nfe2−/− (n = 3 per stage) brains at (A) E11.5, (B) E12.5, (C) E13.5, and (D) E14.5. Sites of ICH are indicated by yellow arrows. TER119 (red), erythrocytes; PECAM1 (white), cerebral vasculature. Scale bars for E11.5 to E13.5, 150 μm; inset, 50 μm; scale bars for E14.5, 200 μm; inset, 50 μm . (E) Summary of hemorrhage types between E11.5 and E12.5 (n = 5) and between E13.5 and E14.5 (n = 8) scored from vibratome cut coronal brain sections. LGE, lateral ganglionic eminence, MGE, medial ganglionic eminence. (F) Light sheet microscopy showing spatial location of hemorrhage in the E16.5 Nfe2−/− embryos, including multiple cerebral bleeds and large ventricle hemorrhage (n = 2). No bleeds can be seen in the E16.5 Nfe2+/+ brain control (n = 2).

ICH first occurs in the ganglionic eminence and progresses to ventricle and cortical involvement. (A-D) Representative confocal images from thick sections of Nfe2+/+ (n = 3 per stage) and Nfe2−/− (n = 3 per stage) brains at (A) E11.5, (B) E12.5, (C) E13.5, and (D) E14.5. Sites of ICH are indicated by yellow arrows. TER119 (red), erythrocytes; PECAM1 (white), cerebral vasculature. Scale bars for E11.5 to E13.5, 150 μm; inset, 50 μm; scale bars for E14.5, 200 μm; inset, 50 μm . (E) Summary of hemorrhage types between E11.5 and E12.5 (n = 5) and between E13.5 and E14.5 (n = 8) scored from vibratome cut coronal brain sections. LGE, lateral ganglionic eminence, MGE, medial ganglionic eminence. (F) Light sheet microscopy showing spatial location of hemorrhage in the E16.5 Nfe2−/− embryos, including multiple cerebral bleeds and large ventricle hemorrhage (n = 2). No bleeds can be seen in the E16.5 Nfe2+/+ brain control (n = 2).

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