Cerebral vasculature development and pericyte recruitment is not disrupted in Nfe2^−/− mice. (A) Coronal brain sections stained with PECAM-1 from E11.5 to E13.5 embryos reveal the stages of blood vessel development in Nfe2^+/+ and Nfe2^−/− mice (n = 3 embryos per genotype per stage; scale bars, 100 μm). (B) Identification (i) and quantification (ii) and of tip cell filipodia in the ganglion eminence of Nfe2^+/+ and Nfe2^−/− mice (n = 3 per genotype). Horizontal bar = mean; error = SD (ii). Scale bars, 50 μm). Data are represented as mean ± SD, and analyzed using Student t test (2-way, unpaired). (C) Pericyte (NG2, white) coverage of the E11.5 cerebral vasculature (PECAM-1, blue) in the head (scale bars, 100 μm) (i) and the ganglionic eminence (scale bars, 50 μm) (n = 3 per genotype) (ii). Inset, optical section of a pericyte in close association with a vessel. (D) Pericyte (NG2, white) coverage of the E14.5 cerebral vasculature (PECAM-1, blue) in the head (scale bars, 100 μm) (i) and ganglionic eminence (scale bars, 50 μm) (ii) (n = 3 per genotype).