Paclitaxel induced phagocytosis-capable TAM populations. (A-B) Volcano plots showing gene expression of BM macrophages from Raji-engrafted RAG2^−/−γc^−/− mice at day 0 (normal), day 4 (early), or day 18 (late) of engraftment. Panels A and B showed the comparison of differential gene expression of day 4 vs day 0 (A) and day 18 vs day 4 (B). (C) Heatmap of M1- and M2-like macrophage marker expression in BM macrophages from Raji-engrafted RAG2^−/−γc^−/− mice at day 0 (normal), day 4 (early) or day 18 (late) of engraftment. (D) UMAP plots of scRNAseq of BM macrophages from Raji-engrafted RAG2^−/−γc^−/− mice treated with control vehicle, aCD47, nab-paclitaxel, or a combination of aCD47 and nab-paclitaxel. (E) Percentages of cells in clusters 0 to 8 analyzed in panel D. (F) Heatmap of representative genes specifically expressed in cluster C2 from scRNAseq clustered in panel D. (G) Expression of genes Pf4, CD209a, Mrc1, Clec10a, and Axl from scRNAseq clustered in panel D. (H) Immunoblots showing the phosphorylation of protein kinases SFK, BTK, MEK, and PI3K in BMDMs during phagocytosis of Raji cells induced by aCD47. (I) A phagocytosis assay with BMDMs and Raji cells; CD47-blocking antibody was given to all conditions; BMDMs were treated with kinase inhibitors as indicated in the figure. ***P < .001 (1-way ANOVA test). (J) Immunoblots showing Src phosphorylation induced by paclitaxel in human THP1 macrophages and mouse BMDMs. UMAP, Uniform Manifold Approximation and Projection.