A combination of paclitaxel and aCD47 remodeled TME of lymphoma by preventing the accumulation of TAM population specifically present in late-stage NHL. (A) Heatmap of representative genes specifically expressed in cluster C1 from scRNAseq clustered in Figure 5D. (B) Heatmap of genes in panel A in BM macrophages from Raji-engrafted RAG2^−/−γc^−/− mice at day 0 (normal), day 4 (early), or day 18 (late) of engraftment, analyzed by RNAseq. (C) Expression of genes Socs3, Sdc4, Mmp14, and Arg2 from scRNAseq clustered in Figure 5D. (D) UMAP plots of scRNAseq of BM macrophages from Raji-engrafted RAG2^−/−γc^−/− mice treated with control vehicle, aCD47, nab-paclitaxel, or a combination of aCD47 and nab-paclitaxel, analyzed by supervised clustering. (E) Percentages of cells in clusters 0 to 4 analyzed in panel D. (F) Heatmap of genes in panel A from scRNAseq clustered in panel D. (G) Expression of genes Socs3, Sdc4, and Mmp14 in macrophages of a cohort of stage 1 to 4 DLBCL patient biopsies, inferred by CIBERSORT; for biopsies with stages 1, 2, 3, and 4, n = 174, 213, 262, and 429. UMAP, Uniform Manifold Approximation and Projection.