Genes responding to LASP treatment in ALL cell lines. (A-B) Venn diagrams of overlap between LASP response genes between sensitive (S, green) and resistant (R, red) ALL cell lines (n = 3 independent experiments per cell line and timepoint). Reproducibly upregulated LASP response genes are provided in (A), and reproducibly downregulated LASP response genes are provided in (B). Overlapping (common) LASP response genes between sensitive and resistant cells are listed. (C) Average gene expression (in transcripts per million) across sensitive (S, green) and resistant (R, red) ALL cell lines before (0h) and after 4 hours (4h) of LASP treatment (1 IU/mL) for reproducibly-induced response genes. Data for ASNS (resistant only), SLC7A11 (common), DDIT3 (common), and ATF3 (sensitive) (n = 4 resistant cells and n = 3 sensitive cells; mean ± SEM). (D) Fold-enrichment in significantly biological pathways from gene ontology analysis of reproducible inducible response genes from resistant and shared (resistant + sensitive) cell lines (FDR <0.05). (E) Significantly enriched TF motifs observed in LASP response genes reproducibly upregulated in resistant-only and shared (resistant + sensitive) cell lines as identified by HOMER (P < .05).