Binding kinetics of PF4-specific Abs from VITT samples in the presence of different anticoagulants. Biotinylated PF4 (5 µg/mL) was immobilized on streptavidin biosensor tips, and binding kinetics of serum samples from patients with VITT (n = 5) were analyzed using BLI. For dissociation, different anticoagulants (heparin, 0.1 U/mL [pink]; heparin, 1 U/mL [dark red]; danaparoid, 0.8 U/mL; fondaparinux 8 µg/mL; argatroban 8 µg/mL) or Octet buffer were applied. Curves and data points represent the mean of technical duplicates (n = 2). (A) Schematic illustration of the experimental setup. (B) Representative binding responses of PF4-specific Abs from a VITT patient serum (VITT1). (C) Quantification of residual binding in percent at time point 560 secondsof the dissociation (percent Residual Binding T_560s) of VITT serum samples.