Leukocyte recruitment is impaired in sterile inflammation in hyperuricemic mice. (A) Alb-creERT2;Glut9^lox/lox mice and Glut9^lox/lox control mice were injected intraperitoneally with tamoxifen. Both groups were fed either an acidogenic diet enriched with inosine or a standard chow diet with inosine for 22 days. On day 21, mice received a subcutaneous injection of monosodium urate (MSU) crystals (5 mg) or vehicle into a preexisting air pouch and were euthanized 12 hours later. (B-C) Serum uric acid (B), blood urea nitrogen (BUN), and creatinine (C) levels of Glut9^lox/lox mice with chow diet and inosine (healthy), Alb-creERT2;Glut9^lox/lox mice with chow diet (HU), or acidogenic diet with inosine (HU plus CKD) on day 22 (n = 7 mice per group) (using 1-way ANOVA). (D) Representative images of vehicle and MSU crystals injected into the air pouch. (E-G) Gating strategy (E) and number (No.) of CD45⁺ leukocytes (F) and neutrophils (G) in air pouch per µL from mice with or without MSU crystals determined by flow cytometry (n = 3-6 mice per group). (H-J) Concentrations of IL-1β (H), IL-6 (I), and CXCL1 (J) measured in the air pouch fluid via ELISA (n = 3-6 mice per group). Data are mean plus or minus SD. *P < .05, **P < .01, ***P < .001. ns, not significant by 1-way ANOVA; ELISA, enzyme-linked immunoassay.