Decreased neutrophil adhesion and extravasation in crystal-stimulated cremaster muscle venules in hyperuricemic mice. (A) Alb-creERT2;Glut9^lox/lox and Glut9^lox/lox control mice were injected with tamoxifen and placed on a chow or acidogenic diet with inosine (n = 5-7 mice per group). HU plus CKD mice received vehicle or rasburicase treatment. After 22 days, mice were injected intrascrotally with MSU crystals (0.5 mg in 200 µL NaCl per mouse) or vehicle (200 µL NaCl per mouse) 4 hours prior to intravital microscopy. (B) Quantification of adhesion efficiency (n = 5-7 mice per group). (C-D) Cumulative distribution (C) and mean leukocyte rolling velocity (µm per second) (D). (E) Quantification of leukocyte rolling depicted as rolling flux fraction. (F) The total number of perivascular neutrophils and mononuclear cells per mm² after MSU crystal injection (n = 5 mice per group). (G) Quantification of the adhesion efficiency (n = 5 mice per group). (H-I) Cumulative frequency over leukocyte rolling velocity (H) and mean of rolling velocity (µm per second) (I). (J) The total number of perivascular neutrophils and mononuclear cells per mm² after MSU crystal injection in vehicle- and rasburicase-treated HU plus CKD mice (n = 4-5 mice per group). Data are mean plus or minus SD. *P < .05, **P < .01, ***P < .001. ns, not significant by Student t test or 1- or 2-way ANOVA.