Hemogen interacts with the SWI/SNF complex and affects H3K27ac enrichment at erythroid promoters and enhancers. (A) Mass spectrometry analysis identifies interaction between hemogen and the SWI/SNF and cohesin complexes. (B) Coimmunoprecipitation (IP) analysis of interactions between hemogen (hem) and SWI/SNF components BRG1, BAF170, and BAF47. ChIP-qPCR for BRG1 (C), BAF170 (D), and BAF47 (E) occupancy in the β-globin locus in hemogen-KO and WT K562 cells. (F) MNase-qPCR showing sensitivity of the β-globin locus to digestion in hemogen-KO and WT K562 cells. (G) Normalized peak density plot showing H3K27ac enrichment in WT and hemogen-KO cells at the TSSs of downregulated genes shown in Figure 2A (n = 3 biological replicates). (H) RNA-seq, H3K27ac, and ATAC-seq signals in the β-globin locus. ATAC-seq data from ENCODE. Dashed box encloses the β-globin LCR. Error bars in (C-F) represent standard deviation. n = 3 biological replicates. *P < .05, **P <.01, 2-tailed Student t test. IgG, immunoglobulin G.