RPF distribution along F9, ACTB, and GAPDH genes. The cumulative sum of normalized and log-transformed RPF density along the F9 (A), ACTB (B), and GAPDH (C) genes in lentiviral-transduced stable expression cell lines of wild-type (WT), OPT-1A, and OPT-2A constructs is shown. FIX domain structure is included in panel A to illustrate the local translation kinetics differences. (A) Distinct RPF profiles for both OPT-1A and OPT-2A variants compared with WT. On the other hand, the RPF profiles in the identical C-terminal V5-His tag regions of F9 constructs (A) and control genes ACTB (B) and GAPDH (C) are similar.