Metabolome of RBCs in stored in SAGM and PAGGGM. Purine (A) and free fatty acid metabolism (B) comparing 35-day stored RBCs in SAGM and 35-day stored RBCs in PAGGGM. The boxplots show metabolite concentrations in RBCs before transfusion. Line plots indicate the median with 25th and 75th percentile of metabolite concentrations over time in the recovered RBCs, up to 1 day after transfusion. *P < .05, **P < .01, and ***P < .0001; NS, not statistically significant.