Inflammatory agent LPS induces AID expression through the NF-κB pathway in BCR-ABL1⁺ B-ALL cells. (A-B) The mRNA and protein expression levels of AID were detected by (A) qRT-PCR and (B) western blotting in BCR-ABL1⁺ B-ALL cells after stimulation with LPS (0, 5, 15, 25 µg/mL) for 24 hours. Gapdh was used to normalize cDNA amounts, and actin was used as the loading control for western blotting. The protein levels were quantified by the ImageJ program and normalized to actin. (C) BCR-ABL1⁺ B-ALL cells were treated with LPS (25 µg/mL) or PBS for 24 hours, the expression of TLR4 was measured by flow cytometry. (D-E) BCR-ABL1⁺ B-ALL cells were treated with LPS (25 µg/mL) or PBS for 24 hours. Western blotting analysis for phospho-specific antibodies against p65, blots were stripped and reprobed with antibodies against total p65. (E) Phosphorylated protein levels were quantified by the ImageJ program and normalized to total form. The protein expression levels of p100 and p52 were detected by western blotting. (D) The protein levels were quantified by the ImageJ program and normalized to actin. The data shown are representative of at least 3 independent experiments. Error bars represent the mean ± standard error of the mean. *P < .05, **P < .01, ***P < .001.