Genetic ablation of platelet PA aggravates inflammatory bleeding. (A) Experimental scheme for acute lung injury in platelet-specific CypD-knockout mice. (B) Representative image of BALF from platelet-specific CypD-knockout mice and Cre-negative littermates. (C) Flow cytometric analysis of RBC and platelet counts in BALF. Student’s t test, two-tailed, unpaired. (D) Representative micrograph of immunofluorescence-stained lung slices from CypD-knockout mice and Cre-negative control animals. Bar represents 100 µm. (E) Histological quantification of alveolar hemorrhage (TER119⁺ area) as well as pulmonary neutrophil and platelet recruitment. Student’s t test, two-tailed, unpaired. (F) Magnified excerpts of representative micrographs, corresponding to white rectangle in Figure 5D. Bar represents 50 µm. (G) Experimental scheme for acute lung injury in TMEM16F-knockout mice. (H) Representative image of BALF from platelet-specific TMEM16F-knockout mice and Cre-negative littermates. (I) Flow cytometric analysis of RBC and platelet counts in BALF. Student’s t test, two-tailed, unpaired. (J) Representative micrograph of immunofluorescence-stained lung slices from TMEM16F-knockout mice and Cre-negative littermates. Bar represents 100 µm. (K) Histopathological quantification of alveolar hemorrhage (TER119⁺ area) as well as pulmonary neutrophil and platelet recruitment. Student’s t test, two-tailed, unpaired. (L) Magnified excerpts of representative micrographs, corresponding to white rectangle in Figure 5J. Bar represents 50 µm.