Signal transduction is carried by RNA-positive platelets. Signal transduction was determined by the percentage of pAKT-AF488–positive cells after stimulation with 5 µg/mL (final) collagen in large and small platelets (A) and RNA-positive and RNA-negative platelets (B) as determined by costaining with T-oligonucleotides and pAKT-AF488 antibody. (C) Maximum pAKT-AF488 positive large and small as well as RNA-positive and RNA-negative platelets after stimulation with 5 µg/mL (final) collagen. Mean ± standard error of mean; n ≥ 4. **P < .01. pAKT, phosphorylated AKT; n.s., not significant.