CD4⁺ T-cell proliferation in response to overlapping mannosylated peptides. (A) Peptides, 15 amino acids in length, overlapping with 2118MP1 were synthesized with high-mannose glycan Man6GlcNAc2 attachments. (B) CD4⁺ T-cell proliferation levels were measured in response to mannosylated peptides MP1 (top left panel), MP2 (top right panel), and MP3 (bottom panel) and their non-glycosylated counterparts (NGP1, NGP2, and NGP3). The data are presented as means with standard deviation from 3 separate experiments (**P < .01; ***P < .001).