Changes in markers of hemolysis based on Hb concentration (A) and absolute reticulocyte counts in the blood (B), and cell-free Hb (C) in the urine of GBT1118 treated vs control (untreated) sickle mice. Urine biomarkers of reactive oxygen species (TBARS) (D), glomerular (nephrin) (E), and tubular injury (KIM-1) (F) in GBT1118-treated control sickle and Hb AA mice. Measures of kidney dysfunction as assessed by 24-hour urine albumin (G), 24-hour urine protein concentration (H), and serum cystatin C (I) in GBT1118-treated sickle, control sickle, and Hb AA mice. Each group contained 10 mice. *P < .05 and **P < .01 for differences between the GBT1118-treated vs control sickle mice.