Glycosyltransferases (GTs) important for sLe^x decorations are differentially expressed between murine Flk2⁻CD34⁻ LT-HSCs and Flk2⁻CD34⁺ ST-HSCs. GT gene expression was assessed using real-time semiquantitative PCR. qPCR was performed as detailed in the Materials and methods section. (A) The level of expression of the murine GT genes, fucosyltransferases (FT), and sialyltransferases (ST) were determined relative to the GADPH housekeeping gene. Each data point represents the mean ± SEM (n = 3 independent experiments) and statistical analysis was performed using the unpaired t test (*P = .033, **P = .002, and ***P < .001). (B) Heatmap generated from qPCR of GT gene expression in the murine HSC populations. Gene expression of the 14 GT genes was determined and hierarchically clustered in a genewise manner according to the correlation distance and average clustering and with the tightest cluster first. ClustViA, a web tool, was used for visualizing the clustering of multivariate data (BETA). The color intensity indicates expression levels where red is the high-expressed and blue is the low-expressed, while gray indicates that it wasn’t detected. Each sample was performed in biological triplicates (n = 3).