Figure 2.
Progenitor characterization of WT and KI-STIM1I115F mice. (A-B) Frequency of the indicated progenitor cells; Mann-Whitney U test (n = 6 per group) P value: ^^≤0.0079 and ^≤0.00224 for WT vehicle vs KI-STIM1I115F vehicle. (C) Representative immunofluorescence staining for STIM1 (green), CD41 (blue), DAPI (cyano), and merged image on megakaryocytes of WT mice (see Supplemental Methods for the other groups). Top panel: Magnification 40 × 1.4 NA (numerical aperture). Bar = 25 μm. Bottom panel: Magnification 63 × 1.4 NA (numerical aperture). Bar = 10 μm. The data are representative of 5 experiments with similar results. (D) Box-and-whisker plots show median and IQR of the mean number of MK colonies/well in WT and KI-STIM1I115F mice. Mann-Whitney U test. P value: ^≤0.0111 WT vehicle vs KI-STIM1I115F vehicle.

Progenitor characterization of WT and KI-STIM1I115F mice. (A-B) Frequency of the indicated progenitor cells; Mann-Whitney U test (n = 6 per group) P value: ^^≤0.0079 and ^≤0.00224 for WT vehicle vs KI-STIM1I115F vehicle. (C) Representative immunofluorescence staining for STIM1 (green), CD41 (blue), DAPI (cyano), and merged image on megakaryocytes of WT mice (see Supplemental Methods for the other groups). Top panel: Magnification 40 × 1.4 NA (numerical aperture). Bar = 25 μm. Bottom panel: Magnification 63 × 1.4 NA (numerical aperture). Bar = 10 μm. The data are representative of 5 experiments with similar results. (D) Box-and-whisker plots show median and IQR of the mean number of MK colonies/well in WT and KI-STIM1I115F mice. Mann-Whitney U test. P value: ^≤0.0111 WT vehicle vs KI-STIM1I115F vehicle.

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