Screening of MYH9-RD mutations reveals that the expression of E1841K mutant inhibits cAMP-induced endothelial VWF secretion. (Ai) Western blots of NMII-A mutants in HUVECs expressing scrambled shRNA (SCR) or shRNA targeting NMII-A (shNMII-A) that were rescued by NMII-A WT and 5 MYH9-RD mutants. (Aii) Ratio of NMII-A to loading control α-tubulin of (Ai). (B) ELISA of VWF secreted from HUVECs in A with forskolin stimulation (n = 12; NS > 0.05; **P < .01; ***P < .001vs WT). NS, not significant.