Figure 6.
Defective cAMP-induced VWF secretion is associated with decreased number of actin framework formation in primary ECs derived from endothelium-specific E1841K mutant mice. (A) VWF secretion in BECs from cre-, PF4-cre, and Tie2-cre/E1841Kflox/flox mice with forskolin stimulation (NS > 0.05; ***P < .001). (B) Western blotting of p-S1916, p-S1943, and total NMII-A in BECs from cre-, PF4-cre, and Tie2-cre/E1841Kflox/flox mice stimulated with forskolin for 15 minutes. (C) Immunostaining of VWF (yellow), NMII-A (magenta), and F-actin (cyan) in BECs from cre-, PF4-cre, and Tie2-cre/E1841Kflox/flox mice. Scale bars represent 2 μm. (D) Quantitative analysis of the number of actin framework-coupled WPBs per cell. (E) The total number of WPBs per cell (n = 16; NS > 0.05; **P < .01). NS, not significant.

Defective cAMP-induced VWF secretion is associated with decreased number of actin framework formation in primary ECs derived from endothelium-specific E1841K mutant mice. (A) VWF secretion in BECs from cre-, PF4-cre, and Tie2-cre/E1841Kflox/flox mice with forskolin stimulation (NS > 0.05; ***P < .001). (B) Western blotting of p-S1916, p-S1943, and total NMII-A in BECs from cre-, PF4-cre, and Tie2-cre/E1841Kflox/flox mice stimulated with forskolin for 15 minutes. (C) Immunostaining of VWF (yellow), NMII-A (magenta), and F-actin (cyan) in BECs from cre-, PF4-cre, and Tie2-cre/E1841Kflox/flox mice. Scale bars represent 2 μm. (D) Quantitative analysis of the number of actin framework-coupled WPBs per cell. (E) The total number of WPBs per cell (n = 16; NS > 0.05; **P < .01). NS, not significant.

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