scRNA-Seq reveals neural crest differentiation pathways. (A) Immunohistochemical staining of E11 wild-type embryo cryosection for Ngfr (green), p57Kip2 (red), and Th (white). Bar represents ventral (V) vs dorsal (D) subdissection. (B) t-SNE plot of remaining 4 clusters after cell cycle correction (left) and colored according to ventral and dorsal origin (right). t-SNE gene expression plots colored for expression levels of SNS genes Sox10 (C), Th (D), Phox2a (E), Gata3 (F), Phox2b (G) and Cdkn1c/p57Kip2 (H).