Notch signaling is downregulated upon SA maturation. (A) t-SNE plot of 4 clusters with slingshot-identified lineage trajectory nodes superimposed. (B) t-SNE gene expression plot colored for expression levels of Ngfr. Pseudotime plots for SNS differentiation markers Sox10 (C), Th (D), Phox2a (E), Gata3 (F), Phox2b (G) and Cdkn1c/p57Kip2 (H). t-SNE gene expression plots colored for expression levels of Notch pathway-associated genes Notch1 (I), Notch2 (J), Notch3 (K), Hes1 (L), Hes6 (M), Dlk1 (N) and Dll3 (O).