Immune profile of primary tumor at day 16. (A) Population of CD8⁺ T cells in tumor-infiltrating lymphocytes (TILs). (B) Population of IFN-γ⁺ cytotoxic T lymphocytes (CTLs) in CD8⁺ population. (C) Population of CD4⁺ T cells in TILs. (D) Population of CD3⁺CD4⁺CD25⁺FoxP3⁺ Tregs in TILs. (E) CD8⁺/CD4⁺ T-cell ratio in TILs. (F) CTL/Treg ratio in TILs to evaluate immunologic balance of tumor after therapy. Data were obtained from at least 5 independent samples. Data are shown as means ± standard deviations. *P < .05, **P < .01, ***P < .001 (2-tailed paired t test).