Systemic immunologic memory response from photothermal ablation combined immunotherapy. (A) Population of CD3⁺CD4⁺CD44^highCD62L^low effector memory CD4 T cells (CD4 Tems) in tumor-infiltrating lymphocytes (TILs) of secondary tumor. (B) Population of CD3⁺CD8⁺CD44^highCD62L^low CD8 Tems in TILs secondary tumor. (C-D) Population of CD4 Tems (C) and CD8 Tems (D) in the spleen. Data were obtained from at least 5 independent samples. Data are shown as means ± standard deviations. *P < .05, **P < .01, ***P < .001 (2-tailed paired t test).