T-cell profiling of splenocytes after CpG deoxynucleotide/PTT therapy. (A-I) Populations of CD4⁺ T cells (A), Th1 T cells (CD4⁺ IFN-γ) (B), Th2 T cells (CD4⁺ IL-4) (C), Th17 T cells (CD4⁺ IL-17) (D), Tregs (CD4⁺CD25⁺FOXP3⁺) (E), CD8⁺ T cells in total T cells (F), IFN-γ–expressing CD8⁺ T cells (G), granzyme-expressing CD8⁺ T cells (H), and dual-expressing CD8⁺ T cells in total T cells (CD45⁺CD3⁺) (I). *P < .05, **P < .01 (2-tailed paired t test). ns, not significant.