Intrinsic characteristics of apheresed T cells and quality of the CAR T-cell product. (A) Representative staining of naïve/stem cell memory (T_N/SCM; CD45RO⁻ CD27⁺), central memory (T_CM; CD45RO⁺CD27⁺), effector memory (T_EM; CD45RO⁺CD27⁻), and effector (T_EFF; CD45RO⁻CD27⁻) CD8 and CD4 subsets in apheresed T lymphocytes. (B) Distribution of T_N/SCM, T_CM, T_EM, and T_EFF CD8 and CD4 T cells in apheresed T lymphocytes from 30 patients treated with tisa-cel. Bars represent the median. (C) Correlation between the naïve/stem cell memory phenotype and in vitro proliferative capacity (proportion of CFSE^low cells) of apheresed CD8 (left panel) and CD4 (right panel) T cells. (D) The CD4/CD8 T-cell ratio in the final product correlates with the ratio of CD4 T_N/SCM to CD8 T_N/SCM cells in the apheresis (left) and the ratio of CFSE^low CD4 to CD8 T cells (right). Pearson correlation test. ρ coefficient (R) and P values are indicated.