Characteristics of apheresed T cells and clinical responses to tisa-cel. (A) Higher frequency of CD8 T_N/SCM, but not of CD4 T_N/SCM, in the starting material from 13 patients with early objective response (OR; ie, partial or complete response) compared with 17 nonresponders (NR; stable or progressive disease) at 1 month (M1). Comparisons between groups based on the Mann-Whitney test. Two-sided P values are indicated. ns, not significant. (B) Higher proportion of CD8 T_N/SCM (left panel) and lower CD4_N/SCM/CD8_N/SCM ratio (right panel) in the starting material from 7 complete responders (CR) compared with 23 NR at 3 months (M3). (C) In vitro proliferative capacity of apheresed CD8 T cells is higher and that of CD4 T cells is lower in CR than in NR at M3. Bars represent the median. (D) Effect of apheresed CD8 T_N/SCM (left panel), CD4 T_N/SCM (middle panel) cells, and CD4 T_N/SCM/CD8 T_N/SCM ratio (right panel) in the starting material on 1-year PFS. P values calculated using the log-rank test. “High” and “low” proportions refer to the 4th and 1st quartiles, respectively. (E) PFS is significantly increased in patients with “high” proportions of proliferating CD8 cells (left panel) and “low” proportions of proliferating CD4 T cells (right panel) in the starting material.