LNs have impaired migratory activity, reduced TNF-α, and increased IL-6 secretion compared with BMNs. (A) Ratio of migrated BMN and LN number by each chemoattractant compared with that of vehicle. (B) mRNA expression of chemotactic receptors in neutrophils. (C) ROS production of neutrophils 30 minutes after PMA stimulation. (D) Inflammatory cytokines from cultured media of sorted neutrophils after 24 hours of vehicle or LPS stimulation. ND, not detected. (E) GSEA of IL-6 production in LNs compared with BNs. (F) IL-6 mRNA expression in RNA-seq. Data are shown as mean ± standard error of the mean. Significant differences are denoted as *P < .05; **P < .01; ***P < .001; ****P < .0001. % of max scale of each channel is presented as a percentage of maximum count (C).