Acetylation contributes to platelet storage lesion. (A) Flow diagram for studying the dynamic changes of the acetylation level of proteins in stored platelets. The 3 × 109 platelets were collected daily from day 0 to day 6. The protein in platelets were digested by trypsin and mixed after tandem mass tag (TMT) labeling. The acetylation peptides were enriched by anti-acetylated lysine antibody conjugated beads and assayed by liquid chromatography/mass spectrometry (LC-MS). (B) The left panel shows the number of proteins in which acetylation levels were upregulated (fold ≥1.3). The right panel shows the subcellular location of the proteins in which acetylation levels were changed during storage (fold ≥1.3). (C) Kyoto Encyclopedia of Genes and Genomes pathway enrichment was performed of the proteins in which acetylation levels were changed during storage (fold ≥1.3). (D) The aggregation, apoptosis, MMP, and mtROS levels were measured in platelets stored for 6 days with 10 µM P300 inhibitor C646 or 10 µM Tip60 inhibitor MG149. **P < .0021, ****P < .0001. n = 3. cGMP-PKG, cyclic guanosine monophosphate–dependent protein kinase G.