lrWGS can be performed directly on denatured cells subjected to FACS from diagnostic bone marrow (BM) samples. (A) Schematic overview of experimental setup. To perform genetic, transcriptional, and epigenetic characterizations, MM and normal cells (T cells [T] or myeloid cells [My]) were subjected to FACS from patient BM samples. lrWGS libraries were prepared directly on denatured cells without prior DNA purification. (B-D) Scatter plot (B) and box plots (C-D) showing the N50 phase block size and average DNA molecule length of lrWGS libraries prepared using the indicated cell type and method. Quality control data from published lrWGS generated from prepared DNA (HMW or column purified) on the 10X platform are shown for comparison.^21,31,50