Single cell RNA-seq reveals gene expression alterations in response treatment. (A) UMAP representation of the complete dataset of 76 862 quality-filtered cells. (B) Myeloblast cells stratified by gilteritinib response (rows) and timepoint relative to starting therapy (columns). Cell color is calculated as the log₁₀-transformed cell count within hexagonal bins. (C) Differential gene expression for gilteritinib-unresponsive patients. Red indicates genes with adjusted P < .1 and fold change ≥1.5; see also supplemental Table 6. (D) Expression of cytokine/chemokines and cytokine receptors pre-and post-treatment in gilteritinib-sensitive and -unresponsive patients. Circle size, fractional proportion of cells expressing a given marker; color scale, scaled expression level.