Genome-wide in vivo CRIPSR screens in 2 murine models of AML. (A) Schematic of the screening strategy. (B) Definition of 3 classes of statistically significant CRISPR hits according to time points compared. (C) For every gene screened, median log2FC of sgRNA representation, in vitro or in vivo as defined in panel B, for each cell line. (D) Numbers of genes that are essential (permutation P < .01) according to screening condition. (E) Overlap (pink cells) between genes identified as essential in our screen and other public screens. (F) Gene set enrichment analysis of genes identified to be essential only in vivo. (G) Gene set enrichment analysis of genes identified to be essential only in vitro or shared in vitro/in vivo. ER, endoplasmic reticulum; mRNA, messenger RNA; TCA, tricarboxylic acid; UTR, untranslated region.