Validation of essential genetic hits using a focused CRISPR screen in vivo. (A) Schematic representation of the gene candidate selection workflow. (B) Schematic of the screening strategy. (C) Numbers of genes that are essential (P < .01) according to screening condition. (D) Specificity of the in vivo effect for every gene as represented by the log2FC in vivo depletion minus the in vitro depletion. Colors indicate genes that are essential (P < .01) according to screening condition. (E) Depletion values for the 72 genes that are essential in vivo (P < .01) for at least one cell line but not essential in vitro.