Essential role for β-galactosylation and immune regulators in vivo. (A) STRING functional protein association network of in vivo–specific hits with at least one connection. (B) For in vivo–only hits with gene ontology annotation “immune system process” (GO:0002376), log2FC depletion values according to condition and cell line. (C) Schematic representation of the galactosyltransferase role of B4galt1. (D) Overall survival of mice transplanted with MA cells after transduction with control sgRNA (sg Ctrl) or sgRNA targeting B4galt1 (2 distinct sgRNAs per group) (E) Immunoblot analysis of total lysates from control or B4galt1^CRISPR-KO MA cells with an antibody against β1 integrin. HSC, hematopoietic stem cell; IFN, interferon; MHC, major histocompatibility complex; NK, natural killer.