Staining of RhoA and RhoB in murine and human MKs. (A) Representative confocal images of in vitro–differentiated MKs 24 hours after bovine serum albumin gradient. The MKs were spun down to Poly-L-Lysine–coated glass slides and stained with Phallodin-Alexa488 (cyan), RhoA-Alexa546 (yellow), RhoB-Alexa647 (magenta), and DAPI to visualize the nucleus. Scale bar, 50 µm. Localization of RhoA and RhoB was analyzed by measuring the signal intensity on the white line (n = 2). (B) Representative confocal image of the human BM. Cryosections were stained with GPIX-FITC, RhoB-A546, and DAPI to visualize the nucleus. Scalebar, 50 µm. DAPI, 4′,6-diamidino-2-phenylindole.