T cells are transcriptionally reprogrammed during TFIs. (A) Principal component analysis. (B) Volcano plot of day 14 TFI vs CONT T cells; P_adj < .05. Selected genes are highlighted as significantly downregulated (blue) or significantly upregulated (red) in TFI vs CONT cells. (C) Heatmap with hierarchical clustering of the top 100 differentially expressed genes in day-14 TFI vs CONT T cells; P_adj < .05. Selected genes are highlighted. (D) Log₂(TPM) expression level of TCF7 and IL7R across timepoints 0, 7, and 14 days in TFI vs CONT T cells. (E) Pathways enriched in day-14 TFI vs CONT T cells; P_adj < .05. (F) Gene set enrichment analysis of day-14 TFI vs CONT T cells using MSigDB and the gene set GSE9650_EFFECTOR_VS_MEMORY_CD8_TCELL_UP.²⁹ Line plots present mean ± SEM values. CONT, continuously; NES, normalized enrichment score; ±SEM, standard error of the mean; TFI(s), treatment-free interval(s).