Neonatal thymectomy has no effect on embryonic-derived Vγ5 and Vγ6 γδ T cells. (A) Schematic diagram of neonatal thymectomy experiment. P1 newborn mice were thymectomized and analyzed 6 weeks later. (B) Frequencies of double-positive CD4/CD8 T cells found in the thymus and perithymic tissue isolated from sham and thymectomized mice, respectively. (C) Number of Vγ5⁺ γδ T cells in the epidermis isolated from 1 ear of sham and thymectomized mice. (D) Number of Vγ6 IL17 γδ T cells, Vγ4 IL17 γδ T cells, and αβ T cells in 1 inguinal lymph node, isolated from sham and thymectomized mice. (E) Number of αβ CD4⁺CD44⁻ T cells in the spleen. *P < .05, ***P < .001, and ****P < .0001 (Student t test). Data are representative of at least 4 mice in each group from 2 independent experiments. Data are depicted as mean plus or minus standard error of the mean (SEM).