Gene-expression analysis of single E9.5 YS Lin⁻CD117⁺ CD41⁺ YFP⁺ and YFP⁻cells. (A) Single-cell multiplex qPCR analyzed by hierarchical clustering of single YFP⁺ (47 cells) and YFP⁻ (10 cells) E9.5 Lin⁻CD117⁺CD41⁺ cells analyzed for the expression of lymphoid-associated (Il7r, Rag1, Rag2, Flt3), myeloid-associated (Cebpa, Cd32, Csf1r, Csf2r, Csf3r, Irf8, Pu1), B-cell–associated (Pax5, Ebf1), E/Mk-associated (EpoR, Gata1, Mpl, Tal1, Vwf, Klf1), and hematopoiesis-associated (Gata2, Myb, Runx1, Kit, Cd34) genes. (B) Number of Il7r⁺ and Il7r⁻ cells within E9.5 YS YFP-expressing cells.