High number of intratumoral T_reg CELLS correlates with high numbers of CD70⁺ cells in SOX11⁺ nodal MCLs, and it is associated with shorter OS of MCL patients. (A) Representative histological sections from SOX11⁺ and SOX11⁻ nodal MCL primary sample stained with specific anti-human CD70 and FOXP3 antibodies (×100) and with double IHC staining with anti-FOXP3 (red) and CD4 (brown); and FOXP3 (brown) and CTLA-4 (red) (×200), insets (×400). Double-positive cells are marked by black arrows, whereas single-positive cells are marked by a black arrowhead. (B) IHC quantification of FOXP3⁺/CD4⁺ T-cell ratio in SOX11⁺ and SOX11⁻ nodal MCLs. (C) IHC quantifications of (FOXP3⁺CTLA4⁺)/CD4⁺ T-cell ratio in SOX11⁺ and SOX11⁻ nodal MCLs. (D-E) Positive correlation between CD70⁺ cells and FOXP3⁺/CD4⁺ T-cell ratio (D) and CD70⁺ cells and (FOXP3⁺CTLA4⁺)/CD4⁺ T-cell ratio (E) in SOX11⁺ (red) and SOX11⁻ (blue) nodal MCLs, quantified by IHC in our series of nodal MCL (E). Graphs show Pearson correlation coefficient (r), P value, and number of cases analyzed (N). (F) Kaplan-Meier curve and Cox regression showing the association of FOXP3⁺/CD4⁺ cell ratio, quantified by IHC in our series of SOX11⁺ nodal MCL, with OS. High values were defined by Maxstat (cutoff point = 0.40). Log-rank test P value, hazard ratio (HR) with 95% confidence interval (CI), and Cox regression P value are shown.