Figure 4.
Detection of eGFP- or tdTomato-tagged iPSC-derived cells by genomic PCR in biopsy specimens collected from BM, colon, and lymph nodes. BM, lymph node, and colon biopsies collected from animal that did not receive any eGFP or tdTomato cells were used as negative control. Peripheral blood mononuclear cells from animal undergoing transplantation with eGFP-transduced HSPCs were used as positive control for animals that received eGFP-tagged iHPs. Day-10 iHPs expressing tdTomato were used as positive control for animals that received iHPs tagged with tdTomato.