Physioxia does not impact the recovery of Tet2−/− HSCs and HPCs. (A-F) A combination of 2 independent experiments is shown; each experiment assessed 3 mice. BM was harvested and processed in a hypoxic chamber (3% O2, 5% CO2, N2) or ambient air (∼21% O2) and analyzed by flow cytometry for HSC and HPC numbers. Numbers of HSCs (A), MPPs (B), CMPs (C), GMPs (D), MEPs (E), and CLPs (F) per femur. (G-H) A combination of 3 independent experiments is shown; each experiment assessed 3 mice. Progenitor cell numbers were analyzed using a functional HPC colony assay examining CFU-GM, BFU-E, and CFU-GEMM number (G) per femur and S-phase percentage (H). Data are presented as mean ± standard error of the mean. *P < .05; **P < .01; ***P < .001; ****P < .0001 when analyzed by 2-way analysis of variance with a post hoc Tukey’s multiple comparison test. CLPs, Common lymphoid progenitors; CMPs, Common myeloid progenitors; GMPs, Granulocyte-macrophage progenitors; MEPs, Megakaryocyte erythrocyte progenitors; ns, not significant.