Transcriptomic changes in WT and Tet2^−/− LSK cells in response to ambient air exposure. Four biological replicates per group. (A) Y-axis shows differential expression (fold change); x-axis shows average normalized expression across all samples. Differential expression values are calculated as physioxia/air (upregulation means gene expression is increased in physioxia compared with ambient air cells; downregulated means gene expression is decreased in physioxia compared with ambient air cells). Red/blue (upregulated/downregulated) points indicate significantly differentially expressed genes (adjusted P < .05). Dashed lines represent a reference point for a ±1.5-fold change in expression on the graph. (B) Y-axis shows differential expression (fold change); x-axis shows average normalized expression across all samples. Differential expression values are calculated as Tet2^−/−/WT (upregulation means upregulated by Tet2^−/−; downregulated means downregulated by Tet2^−/−). Red/blue (upregulated/downregulated) points indicate significantly differentially expressed genes (adjusted P < .05). Dashed lines represent a reference point for a ±1.5-fold change in expression on the graph. (C) Fast gene set enrichment analysis (FGSEA) is shown for differential expression analysis comparing physioxia isolation to ambient air exposure. ES, enrichment score; GO, Gene Ontology; n, number of genes in gene set and ranking list; NES, normalized enrichment score; P_adj, adjusted P value for FGSEA; rank 1, gene most significantly upregulated by physioxia; rank ∼12 500, gene most significantly downregulated by physioxia.