Whole-genome CRISPR knock-out depletion screen identifies genes differentially required for the growth of mutant CALR-transformed BA/F3-MPL cells. (A) Experimental setup of the whole-genome CRISPR depletion screen on BA/F3 cells. n = 2 independent biological replicates each. (B) Growth curve of BA/F3-MPL cells after splitting into 2 culturing conditions at day 7 (+IL3/−IL3). (C) Volcano plot depicting significance and fold change of depleted genes, separated by the conditions stated, highlighting the most significant depleted genes for each condition. Log₂ fold change threshold = ±1. FDR adjusted P < .05. (D) Genes involved in protein glycosylation among the 10 most significantly depleted genes comparing CALR^Δ52 −IL3 vs EV +IL3 are shown, ranked by log₂ fold change. FDR, false discovery rate; neg. LFC, negative log₂ fold change; LV, lentivirus.