Persistent alterations in the immunoregulatory landscape at M12 after allo-HSCT. (A-B) Frequencies of cell metaclusters (FlowSOM) among PBMCs from patients at M12 after transplantation were compared with those of healthy donors. Six of 18 cell MCs were significantly decreased (A) and 1 was increased (B). Mann-Whitney tests: *P < .05; **P < .01; ***P < .001; ****P < .0001. (C) Each immune cell subset (percentage of cell subset among total CD45⁺ live cells or among a parent cell subset when indicated by "/subset") assessed by classic manual gating was also compared between patients at M12 and healthy donors. Significant (Mann-Whitney test) parameters are annotated. (D) Altered expression of immunoregulatory molecules in indicated T- and NK-cell subsets. Circles indicate significantly increased (red) or decreased (gray) median expression of the regulatory molecules in patients at M12 after HSCT, compared with healthy donors.