MH-driven transcriptional deregulatory activity in B cells. (A) Genes deregulated in pre–pro-B, pro-B, and pre-B cells. Upregulated or downregulated genes are indicated by red and blue bars, respectively. The raw scatter plot is shown in supplemental Figure 3A. (B) Venn diagram indicating the overlap genes upregulated in pre–pro-B, pro-B, and pre-B cells. (C) Venn diagram indicating the overlap genes downregulated in pre–pro-B, pro-B, and pre-B cells. (D) Heatmap representation of MH modulated genes at different B-cell developmental stages in WT and MH-Vav mice (MH). The representative genes are labeled on the right of the heatmap. (E-F) GO analysis of MH-driven gene deregulations in pro-B cells. (G) Scatter plot of gene expression differences between sorted B220⁺CD19⁺IgM ⁺ spleen cells of WT and MH mice at 12 months. The normalized expression of each deregulated gene is shown in dots. (H) Quantitative RT-PCR validation of transcript levels of a number of deregulated genes in WT and MH mice. (I) Circos plot of the V_HJ_H rearrangement in WT (n = 3) and MH (n = 3) mice at 12 months. Ribbon widths are proportional to the frequencies of rearrangement events. Diversity of the V_HJ_H rearrangement in WT and MH mice is summarized in the far-right panel. Data are presented as mean ± standard error of the mean and were analyzed by using the t test. **P < .01, ***P < .001. RPM, reads per million.