The regulatory role of the C-terminal moiety of MH fusion. (A) Schematic diagram of the construction of deletional mutations in the C-terminal MEF2D-HNRNPUL1. The amino acid positions used in this assay are arrowed and annotated. (B) Luciferase assay of MH fusion and its mutants using the HDAC9 promoter. (C) Quantitative RT-PCR of the messenger RNA levels of representative target genes in Reh cells expressing MH or its mutants. (D) Venn diagram indicating the overlap proteins that interacted with MEF2D, MH, and MH-C protein in an MS assay. (E) GO analysis of proteins with unique interaction to MH vs MEF2D in an MS assay. (F) Mammalian two-hybridization assay showing the interaction of MEF2D, MH, and MH-C with HDAC9 and P300-TAZ2 domain in 293T cells. (G) Mammalian two-hybridization assay showing the dimerization of MEF2D, MH-N, MH-C, and MH in 293T cells. Data are presented as mean ± standard error of the mean and are analyzed by using the t test. *P < .05, **P < .01, ***P < .001. VEGFA-VEGFR2, vascular endothelial growth factor A/vascular endothelial growth factor receptor-2.