Phenotype and function of EBER^POS T and NK cells from patient 5. FlowRNA analysis of EBER^POS and EBER^NEG CD4⁺ and CD8⁺ T cells from patient 5. PBMC were stained for CCR7 and CD45RA (A,E), Ki-67 and CD38 (B,F), Foxp3 and CD25 (C,G), and TNF-α and IFN-γ (D,H). Age-matched healthy donors were also analyzed; 1 representative donor is shown. The phenotype and function of the EBER^POS CD4⁺ T cells closely resembled that of the EBER^NEG CD4⁺ T cells and the healthy donors. The EBER^POS CD8⁺ T cells were more activated and all expressed IFN-γ and TNF-α. Total NK cells from patient 5 were stained for CD45RA and CCR7 I) (C), Ki-67 and CD38 (J), and TNF-α and IFN-γ (K). Analysis of NK cells from 1 representative healthy donor is shown. All the EBER^POS NK cells were highly activated and replicative, and they all expressed IFN-γ.